Open Access Brief report

Use of linseed oil to treat experimentally induced keratoconjunctivitis sicca in rabbits

Magda Luzia Neves1, Letícia Yamasaki2, Osimar de Carvalho Sanches2, Marcelo Sávio Paiva do Amaral3, Helaine Stevanin3, Rogério Giuffrida4, Eudes Ramalho Candido4, Jonatas Eduardo Góes4, Luís Felipe da Costa Zulim4, Augusto Schweigert5, Rosimery Missuzu Fukui5, Carlos Collares Meirelles5, Carolina Akemi Sasaki5 and Silvia Franco Andrade6*

Author Affiliations

1 Post-Graduate Program in Animal Science, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

2 Department of Anatomy Pathology, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

3 Department of Small Animal Surgery, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

4 Faculty of Veterinary, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

5 Veterinary Hospital, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

6 Department of Small Animal Medicine, University of Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, Presidente Prudente, São Paulo, 19001-970, Brazil

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Journal of Ophthalmic Inflammation and Infection 2013, 3:4  doi:10.1186/1869-5760-3-4

Published: 3 January 2013

Abstract

Background

The objective of this study was to evaluate the effectiveness of various linseed oil (LO) preparations (oral, topical, oral and topical combined) in treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty male New Zealand white rabbits were divided into four groups: group C (control), group OLO (oral LO), group TLO (topical LO), and group OTLO (oral and topical LO). The animals were evaluated weekly using Schirmer's tear test (STT), fluorescein test (FT), and Rose Bengal test (RBT) and were euthanized at the end of the experiment for histopathological analysis.

Findings

There were significant improvements in the parameters analyzed (STT, FT, and RBT) and in the histopathological finding in all of the groups using LO.

Conclusions

The analyzed results demonstrate that LO, administered orally or topically, was effective in treating experimentally induced KCS in rabbits, although combined oral and topical LO did not show additional benefits greater than those with a single route of administration.

Keywords:
Histopathology; Keratoconjunctivitis sicca; Linseed oil; Omega 3 and 6; Rabbits